Journal: Frontiers in Immunology

Article Title: A specific immune signature for predicting the prognosis of glioma patients with IDH1-mutation and guiding immune checkpoint blockade therapy

doi: 10.3389/fimmu.2022.1001381

Figure Lengend Snippet: The hazard rate of genes for glioma patients with IDH1mt.

Article Snippet: Primary antibodies used were as follows: HLA-DQA2 (1:200; Cat No. 42-669, ProSci, Fort Collins, CO, USA), HOXA3 (1:100; Cat No.ab230879, Abcam, Cambridge, UK), SAA2 (1:500; Cat No. CAU25292; Biomatik, Kitchener, Canada), CD8 (1:4000; Cat No. 66868-1-Ig; Proteintech, Wuhan, China), and CD86 (1:250; Cat No. ab220188, Abcam).

Techniques:

Journal: Frontiers in Immunology

Article Title: A specific immune signature for predicting the prognosis of glioma patients with IDH1-mutation and guiding immune checkpoint blockade therapy

doi: 10.3389/fimmu.2022.1001381

Figure Lengend Snippet: Hub genes selected to construct the risk model. (A, B) LASSO analysis for hub genes associated with the survival rate of IDH1mt-glioma patients. (C) Multivariate Cox regression analysis of HLA-DQA2 , HOXA3 , and SAA2. These three genes were used to construct the risk model.

Article Snippet: Primary antibodies used were as follows: HLA-DQA2 (1:200; Cat No. 42-669, ProSci, Fort Collins, CO, USA), HOXA3 (1:100; Cat No.ab230879, Abcam, Cambridge, UK), SAA2 (1:500; Cat No. CAU25292; Biomatik, Kitchener, Canada), CD8 (1:4000; Cat No. 66868-1-Ig; Proteintech, Wuhan, China), and CD86 (1:250; Cat No. ab220188, Abcam).

Techniques: Construct

Journal: Frontiers in Immunology

Article Title: A specific immune signature for predicting the prognosis of glioma patients with IDH1-mutation and guiding immune checkpoint blockade therapy

doi: 10.3389/fimmu.2022.1001381

Figure Lengend Snippet: Expression of HLA-DQA2 , HOXA3 , SAA2 , CD86 , and CD8 in IDH1mt-glioma tissues. IDH1mt-glioma tissues were divided into long- and short-term survival groups according to the patient’s number of days of survival with the cut-off as 15 months. (A) The IHC score of HLA-DQA2 , HOXA3 , and SAA2 in IDH1mt-glioma tissues in long- and short-term groups. (B) Representative figures of expression of HLA-DQA2 , HOXA3 , and SAA2 in long- and short-term group IDH1mt-glioma tissues. (C) Expression of CD86 and CD8 in long- and short-term group IDH1mt-glioma tissues. (D–F) The diagnostic value of HLA-DQA2 , HOXA3 , and SAA2 for distinguishing long- and short-term survival of IDH1mt-glioma patients. ** P < 0.01.

Article Snippet: Primary antibodies used were as follows: HLA-DQA2 (1:200; Cat No. 42-669, ProSci, Fort Collins, CO, USA), HOXA3 (1:100; Cat No.ab230879, Abcam, Cambridge, UK), SAA2 (1:500; Cat No. CAU25292; Biomatik, Kitchener, Canada), CD8 (1:4000; Cat No. 66868-1-Ig; Proteintech, Wuhan, China), and CD86 (1:250; Cat No. ab220188, Abcam).

Techniques: Expressing, Diagnostic Assay

Journal: International Journal of Molecular Sciences

Article Title: Antibody-Dependent Cytotoxicity of Monocytes in Preeclampsia Is Associated with Soluble Forms of HLA

doi: 10.3390/ijms262311638

Figure Lengend Snippet: ( a )—CD16+ monocytes obtained one day after delivery from a patient with PE; ( b )—the same monocytes in the placental villus-conditioned culture medium (PVCM) for 3 h; ( c )—the same monocytes in the PVCM and the recombinant histocompatibility antigen HLA-B for 3 h.

Article Snippet: The third well contained PVCM and recombinant human HLA class I histocompatibility antigen, B alpha chain (HLA-B), partial, Code CSB-YP355776HU (Cusabio Biotech, Wuhan, China) at a concentration of 10 μg/mL.

Techniques: Recombinant

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway.

doi: 10.1186/s10020-023-00690-x

Figure Lengend Snippet: Fig. 2 A Spleen index of the seven groups of mice. B Immunohistochemical staining for HLA-DR. Scale bars represent 50 μm. Data are expressed as the mean ± SEM (n = 3). Significance levels are indicated as follows: ns, not significant; *p < 0.05, **p < 0.01, #p < 0.05, ##p < 0.01, &p < 0.05, &&p < 0.01

Article Snippet: Paraffin sections were incubated overnight at 4 °C with antibodies against HLA-DR (BOSTER, CA, USA) and Caspase-3 (Cell Signaling Technology, Boston, MA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: Molecular Characteristics of High-Dose Melphalan Associated Oral Mucositis in Patients with Multiple Myeloma: A Gene Expression Study on Human Mucosa

doi: 10.1371/journal.pone.0169286

Figure Lengend Snippet: Genes altered in the buccal mucosa of patients with multiple myeloma that displayed mild/no mucositis (NM) compared to those that displayed ulcerative mucositis (UM).

Article Snippet: Following an in-house optimized protocol, tissues were stained with an antibody against the HLA class II Histocompatibility antigen, DR beta 5 chain (HLA-DRB5 center region) with a rabbit polyclonal antibody (no. OAAB06426, Aviva Systems Biology, CA, US).

Techniques: